Javier Zanetti has made the most career appearances by a player 25 or older in Serie A, with 522 appearances.

most games after turning 25 years old by a player

	NAME	M	MIN	START	G	G/M	A	G+A	PK
1	Javier Zanetti J. Zanetti	522	45,116	500	7	0.01	6	13	0
2	Samir Handanovič S. Handanovič	490	43,854	490	0	0.00	0	0	0
3	Dino Zoff D. Zoff	485	43,624	485	0	0.00			0
4	Gianluca Pagliuca G. Pagliuca	477	42,800	477	0	0.00			0
5	Fabio Quagliarella F. Quagliarella	463	31,430	358	159	0.34	34	193	32
6	Enrico Albertosi E. Albertosi	462	41,100	461	0	0.00			0
7	Pietro Vierchowod P. Vierchowod	449	39,361	444	33	0.07			0
8	Andrea Consigli A. Consigli	443	39,650	443	0	0.00	0	0	0
9	Antonio Di Natale A. Di Natale	441	33,587	385	207	0.47	27	234	33
10	Gianluigi Buffon G. Buffon	437	38,870	437	0	0.00	0	0	0
11	Paolo Maldini P. Maldini	420	36,446	414	16	0.04			0
12	Francesco Totti F. Totti	412	31,557	365	193	0.47	46	239	58
13	Sergio Pellissier S. Pellissier	411	26,511	284	104	0.25	19	123	17
14	Antonio Candreva A. Candreva	410	30,572	360	78	0.19	75	153	21
15	Francesco Antonioli F. Antonioli	401	35,848	401	0	0.00	0	0	0
16	Morgan De Sanctis M. De Sanctis	400	35,894	400	0	0.00	1	1	0
17	Luciano Castellini L. Castellini	396	34,758	396	0	0.00			0
18	Francesco Acerbi F. Acerbi	391	33,647	375	23	0.06	9	32	1
19	Luca Marchegiani L. Marchegiani	389	33,853	382	0	0.00			0
20	Tarcisio Burgnich T. Burgnich	382	34,228	382	5	0.01			0
21	Alessandro Costacurta A. Costacurta	377	31,514	352	2	0.01			1
22	Massimo Gobbi M. Gobbi	365	28,109	312	9	0.02	17	26	0
23	Dario Dainelli D. Dainelli	359	29,575	338	11	0.03	2	13	0
24	Nils Erik Liedholm N. Erik Liedholm	359	32,310	359	81	0.23			25
25	Rodrigo Palacio R. Palacio	357	25,804	288	93	0.26	51	144	4