Azitra (AZTR) had the highest return between 2000 and 2023 by a nano-cap US stock, returning 3,285,614.3%.

ASSET	YEARS	% RETURN
Azitra (AZTR)	2000-2023	3,285,614.29%
Nvni Group Limited Ordinary Shares (NVNI)	2000-2023	1,489,900%
GreenPower Motor (GP)	2000-2023	869,344.44%
Cemtrex (CETX)	2000-2023	865,954.23%
Blackboxstocks (BLBX)	2000-2023	165,782.35%
Future Fintech (FTFT)	2000-2023	146,300%
BioNexus Gene Lab Corp Common stock (BGLC)	2000-2023	129,168.29%
India Globalization Capital (IGC)	2000-2023	46,566.67%
Eastside Distilling (BLNE)	2000-2023	38,638.74%
Foremost Lithium Resource & Technology Ltd Common stock (FMST)	2000-2023	3,377.05%
AquaBounty Technologies (AQB)	2000-2023	1,879.17%
GeoVax Labs (GOVX)	2000-2023	1,833.93%
Gulf Resources (GURE)	2000-2023	1,550%
TNF Pharmaceuticals (TNFA)	2000-2023	1,531.15%
Cross Timbers Royalty Trust (CRT)	2000-2023	1,384.19%
Tandy Leather Factory (TLF)	2000-2023	1,220.05%
NextTrip (NTRP)	2000-2023	1,109.16%
Avalon GloboCare (ALBT)	2000-2023	935%
BioLineRx (BLRX)	2000-2023	914.36%
Jewett-Cameron Trading (JCTC)	2000-2023	877.32%
Bone Biologics (BBLG)	2000-2023	561.46%
Alpha Pro Tech (APT)	2000-2023	537.35%
PetMed Express (PETS)	2000-2023	503.79%
Euro Tech (CLWT)	2000-2023	496.31%
Old Market Capital (OMCC)	2000-2023	480.81%

Nano-cap stocks are treated as having a market cap of <= $50M.