Between the 1999-00 and 2008-09 seasons, Javier Zanetti made the most appearances in Serie A, with 337 appearances.

Most appearances by a player 1999-00 to 2008-09

	NAME	M	MIN	START	G	G/M	PK
1	Javier Zanetti J. Zanetti	337	29,461	325	4	0.01	0
2	Sébastien Frey S. Frey	318	28,539	317	0	0.00	0
3	Simone Perrotta S. Perrotta	304	23,936	277	33	0.11	0
4	Lorenzo Stovini L. Stovini	293	25,981	290	4	0.01	0
5	Clarence Seedorf C. Seedorf	287	21,250	244	44	0.15	0
6	Aimo Stefano Diana A. Stefano Diana	281	21,603	246	24	0.09	0
7	Alberto Gilardino A. Gilardino	281	18,973	207	113	0.40	9
8	Gennaro Gattuso G. Gattuso	276	20,972	243	7	0.03	0
9	Gianluigi Buffon G. Buffon	276	24,686	276	0	0.00	0
10	Martin Jørgensen M. Jørgensen	275	19,358	220	38	0.14	0
11	Iván Córdoba I. Córdoba	275	23,706	266	15	0.05	0
12	Andrea Pirlo A. Pirlo	275	21,171	234	37	0.13	14
13	Pavel Nedvěd P. Nedvěd	273	22,230	259	54	0.20	0
14	Francesco Totti F. Totti	272	23,265	268	142	0.52	40
15	Alessandro Del Piero A. Del Piero	268	19,526	228	118	0.44	35
16	Dejan Stanković D. Stanković	262	19,693	223	39	0.15	0
17	Fabio Liverani F. Liverani	255	18,582	223	10	0.04	1
18	Christian Panucci C. Panucci	255	21,405	241	21	0.08	0
19	Nicola Amoruso N. Amoruso	254	18,220	198	81	0.32	16
20	Max Tonetto M. Tonetto	252	20,782	237	11	0.04	0
21	Gianluca Zambrotta G. Zambrotta	251	20,047	222	8	0.03	0
22	Salvatore Lanna S. Lanna	250	21,440	241	4	0.02	0
23	Simone Vergassola S. Vergassola	249	21,322	237	20	0.08	1
24	Cristian Zenoni C. Zenoni	249	19,278	213	4	0.02	0
25	Paolo Maldini P. Maldini	244	21,040	238	8	0.03	0