The Houston Astros have the worst record against the run line by a team, with a run-line record of 560-558.

which team is worse against the run line, astros or braves
AstrosBraves560-558549-522
TEAMGATSATS COV %ATS AVGO/U RECO/U AVGW%WLABRH2B3BHRRBISHSFSBCSBBIBBHBPSOGIDPPATBXBHAVGOBPSLGOPS
Houston AstrosHouston Astros1118560-55850.1+0.1510-554-54+0.4.60667844038,1485,6099,9172,0491371,5535,391943165321983,8651694338,44593642,85616,8993,739.260.332.443.775
Atlanta BravesAtlanta Braves1071549-52251.3+0.3524-499-48+0.5.53657449736,5545,0829,3031,9301501,3474,8722322545151873,4922834219,11572440,95315,5743,427.255.325.426.751
StatMuse has team, game-level data for run-line records going back to the 2016 season.
Some of the information used here was obtained free of charge from Retrosheet.