The Houston Astros have the worst record against the run line by a team, with a run-line record of 467-476.

which team is worse against the run line, astros or braves
AstrosBraves467-476466-439
TEAMGATSATS COV %ATS AVGO/U RECO/U AVGW%WLABRH2B3BHRRBISHSFSBCSBBIBBHBPSOGIDPPATBXBHAVGOBPSLGOPS
Houston AstrosHouston Astros943467-47649.5+0.1447-454-42+0.5.59556138232,2844,8198,4701,7451241,3224,624832724441743,3021493627,14880936,30314,4293,191.262.335.447.782
Atlanta BravesAtlanta Braves905466-43951.5+0.3441-424-40+0.6.52247243330,9174,2807,8861,6261391,0994,1062312184271563,0112703547,57462034,73113,0872,864.255.326.423.749
StatMuse has team, game-level data for run-line records going back to the 2016 season.
Some of the information used here was obtained free of charge from Retrosheet.