Protagonist Therapeutics (PTGX) had the lowest return in April 2022 by a mid-cap US stock, returning -61.9%.

ASSET	MONTH	% RETURN
Protagonist Therapeutics (PTGX)	April 2022	-61.87%
Terawulf (WULF)	April 2022	-55.15%
Riot Blockchain (RIOT)	April 2022	-50.78%
Eos Energy Enterprises (EOSE)	April 2022	-49.04%
CleanSpark (CLSK)	April 2022	-47.53%
Terns Pharmaceuticals (TERN)	April 2022	-46.67%
Scholar Rock (SRRK)	April 2022	-45.95%
Rhythm Pharmaceuticals (RYTM)	April 2022	-45.52%
Kingsoft Cloud Holdings (KC)	April 2022	-45.23%
Dave (DAVE)	April 2022	-44.14%
Twist Bioscience (TWST)	April 2022	-41.74%
Quantum Computing (QUBT)	April 2022	-41.15%
Hycroft Mining (HYMC)	April 2022	-41%
Adaptive Biotechnologies (ADPT)	April 2022	-40.9%
American Bitcoin (ABTC)	April 2022	-40.87%
Olema Pharmaceuticals (OLMA)	April 2022	-40.42%
TMC the metals (TMC)	April 2022	-39.61%
Spyre Therapeutics (SYRE)	April 2022	-38.98%
10X Genomics (TXG)	April 2022	-37.42%
Century Aluminum (CENX)	April 2022	-36.82%
Hut 8 Corp Common Stock (HUT)	April 2022	-35.74%
Immunitybio (IBRX)	April 2022	-35.64%
Beam Therapeutics (BEAM)	April 2022	-35.36%
Sunrun (RUN)	April 2022	-35.13%
Peloton Interactive (PTON)	April 2022	-33.91%

Mid-cap stocks are treated as having a market cap between $2B and $10B.