The 2023-24 defensemen for the Sabres included Jacob Bryson, Bowen Byram, Joseph Cecconi, Kale Clague and Connor Clifton.

	NO.	NAME	POS	HEIGHT	WEIGHT	DOB	AGE	BIRTHPLACE
1	78	Jacob Bryson J. Bryson	D	5'9"	174	11/18/1997	26	London, Canada
2	4	Bowen Byram B. Byram	D	6'1"	190	6/13/2001	22	Cranbrook, Canada
3	3	Joseph Cecconi J. Cecconi	D	6'3"	210	5/23/1997	26	Youngstown, NY
4	38	Kale Clague K. Clague	D	6'0"	190	6/5/1998	25	Regina, Canada
5	75	Connor Clifton C. Clifton	D	5'11"	190	4/28/1995	28	Matawan, NJ
6	26	Rasmus Dahlin R. Dahlin	D	6'3"	202	4/13/2000	23	Lidkoping, Sweden
7	4	Jeremy Davies J. Davies	D	5'11"	190	12/4/1996	27	Sainte-Anne-de-Bellevue, Canada
8	68	Brandon Fortunato B. Fortunato	D	5'11"	170	6/7/1996	27	Albertson, NY
9	44	Chris Jandric C. Jandric	D	5'11"	180	10/3/1998	25	Prince George, Canada
10	6	Erik Johnson E. Johnson	D	6'4"	225	3/21/1988	35	Bloomington, MN
11	33	Ryan Johnson R. Johnson	D	6'1"	195	7/24/2001	22	Newport Beach, CA
12	10	Henri Jokiharju H. Jokiharju	D	6'0"	200	6/17/1999	24	Oulu, Finland
13		Sean Keohane S. Keohane	D	6'4"	182	11/4/2004	19	Boston, MA
14	76	Vsevolod Komarov V. Komarov	D	6'3"	198	1/11/2004	20	Chelyabinski, Russia
15	83	Mats Lindgren M. Lindgren	D	6'0"	184	8/26/2004	19	North Vancouver, Canada
16		Gavin McCarthy G. McCarthy	D	6'2"	185	6/2/2005	18	Troy, NY
17	73	Zach Metsa Z. Metsa	D	5'9"	192	10/19/1998	25	Delafield, WI
18	91	Nikita Novikov N. Novikov	D	6'4"	209	7/25/2003	20	Moscow, Russia
19	74	Norwin Panocha N. Panocha	D	6'2"	189	2/24/2005	18	Bayreuth, Germany
20	25	Owen Power O. Power	D	6'6"	218	11/22/2002	21	Mississauga, Canada
21	5	Ethan Prow E. Prow	D	5'11"	182	11/17/1992	31	Sauk Rapids, MN
22	23	Mattias Samuelsson M. Samuelsson	D	6'4"	231	3/14/2000	23	Philadelphia, PA
23	45	Nicholas Savoie N. Savoie	D	6'0"	185	5/30/2002	21	Moncton, Canada
24	61	Riley Stillman R. Stillman	D	6'1"	196	3/9/1998	25	Peterborough, Canada
25		Maxim Strbak M. Strbak	D	6'2"	205	4/13/2005	18	Kosice, Slovakia