The 2023-24 defensemen for the Senators included Matthew Andonovski, Jacob Bernard-Docker, Erik Brännström, Thomas Chabot and Jakob Chychrun.

	NO.	NAME	POS	HEIGHT	WEIGHT	DOB	AGE	BIRTHPLACE
1		Matthew Andonovski M. Andonovski	D	6'2"	200	3/14/2005	18	Markham, Canada
2	24	Jacob Bernard-Docker J. Bernard-Docker	D	6'0"	190	6/30/2000	23	Canmore, Canada
3	26	Erik Brännström E. Brännström	D	5'10"	185	9/2/1999	24	Eksjo, Sweden
4	72	Thomas Chabot T. Chabot	D	6'2"	195	1/30/1997	27	Sainte-Marie, Canada
5	6	Jakob Chychrun J. Chychrun	D	6'2"	220	3/31/1998	25	Boca Raton, FL
6	56	Jorian Donovan J. Donovan	D	6'1"	182	4/5/2004	19	Calgary, Canada
7	50	Maxence Guenette M. Guenette	D	6'1"	181	4/28/2001	22	L'Ancienne-Lorette, Canada
8		Tomas Hamara T. Hamara	D	6'0"	185	3/9/2004	19	Prague, Czech Republic
9	23	Travis Hamonic T. Hamonic	D	6'1"	200	8/16/1990	33	St. Malo, Canada
10	29	Dillon Heatherington D. Heatherington	D	6'4"	215	5/9/1995	28	Calgary, Canada
11	43	Tyler Kleven T. Kleven	D	6'4"	200	1/10/2002	22	Fargo, ND
12	32	Jacob Larsson J. Larsson	D	6'2"	193	4/29/1997	26	Ljungby, Sweden
13	61	Ryan MacKinnon R. MacKinnon	D	6'0"	190	9/5/1994	29	Summerside, Canada
14	33	Nikolas Matinpalo N. Matinpalo	D	6'3"	207	10/5/1998	25	Espoo, Finland
15	85	Jake Sanderson J. Sanderson	D	6'3"	195	7/8/2002	21	Whitefish, MT
16	37	Donovan Sebrango D. Sebrango	D	6'1"	194	1/12/2002	22	Ottawa, Canada
17		Hoyt Stanley H. Stanley	D	6'2"	187	2/4/2005	18	West Vancouver, Canada
18	60	Lassi Thomson L. Thomson	D	6'0"	190	9/24/2000	23	Tampere, Finland
19		Djibril Toure D. Toure	D	6'7"	203	6/5/2003	20	Montreal, Canada
20	2	Artem Zub A. Zub	D	6'2"	200	10/3/1995	28	Khabarovsk, Russia