Since 2020, the Colorado Rockies have lost the most games by a team, with 538 losses.

Interpreted as:

most regular-season losses by an MLB team since 2020

	TEAM	L	W	L%	G	W%	AB	R	H	2B	3B	HR	RBI	BB	HBP	SO	SB	CS	PA	TB	XBH	SH	SF	IBB	GIDP	AVG	OBP	SLG	OPS
1	Rockies	538	331	.619	869	.381	29,329	3,712	7,242	1,474	177	896	3,563	2,400	287	7,920	411	147	32,348	11,758	2,547	116	216	79	646	.247	.308	.401	.709
2	Nationals	516	354	.593	870	.407	29,105	3,667	7,279	1,440	124	831	3,488	2,529	384	6,695	646	219	32,352	11,460	2,395	127	207	99	671	.250	.316	.394	.710
3	Pirates	505	365	.580	870	.420	28,857	3,359	6,692	1,305	139	777	3,201	2,743	286	7,738	503	169	32,203	10,606	2,221	111	206	86	547	.232	.303	.368	.670
4	White Sox	499	371	.574	870	.426	29,276	3,583	7,087	1,374	69	904	3,433	2,423	319	7,420	396	114	32,294	11,311	2,347	97	179	55	656	.242	.305	.386	.692
5	Athletics	493	377	.567	870	.433	28,907	3,546	6,718	1,372	101	993	3,390	2,709	378	7,666	519	131	32,304	11,271	2,466	106	204	66	599	.232	.305	.390	.694
6	Angels	486	384	.559	870	.441	29,100	3,687	6,879	1,268	120	1,087	3,529	2,636	390	7,990	470	169	32,404	11,648	2,475	94	184	124	583	.236	.307	.400	.707
7	Royals	481	389	.553	870	.447	29,132	3,636	7,171	1,409	174	861	3,497	2,328	313	6,725	685	207	32,157	11,511	2,444	125	259	71	516	.246	.306	.395	.701
8	Marlins	478	392	.549	870	.451	29,240	3,484	7,115	1,329	123	832	3,316	2,392	319	7,463	628	180	32,254	11,186	2,284	105	198	89	624	.243	.306	.383	.688
9	Rangers	471	399	.541	870	.459	29,329	3,804	7,029	1,320	101	1,011	3,627	2,631	300	7,402	593	161	32,519	11,584	2,432	55	204	81	519	.240	.307	.395	.702
10	Diamondbacks	466	404	.536	870	.464	29,275	4,073	7,199	1,493	186	966	3,876	2,903	373	7,095	575	147	33,003	11,962	2,645	171	281	92	575	.246	.319	.409	.728
11	Reds	452	418	.520	870	.480	28,917	3,875	6,940	1,381	123	1,007	3,698	2,823	460	7,763	625	189	32,497	11,588	2,511	96	201	62	593	.240	.316	.401	.716
12	Tigers	451	417	.520	868	.480	29,023	3,604	6,913	1,282	188	876	3,462	2,512	287	7,882	376	118	32,102	11,199	2,346	50	230	51	550	.238	.303	.386	.689
13	Twins	444	426	.510	870	.490	29,255	3,892	7,118	1,443	105	1,104	3,706	2,791	442	7,618	371	114	32,753	12,083	2,652	50	215	75	632	.243	.317	.413	.730
14	Orioles	443	427	.509	870	.491	29,353	3,877	7,163	1,465	127	1,052	3,717	2,576	359	7,544	501	154	32,577	12,038	2,644	72	217	70	519	.244	.311	.410	.721
15	Cubs	433	437	.498	870	.502	29,089	3,975	7,058	1,361	153	1,032	3,793	2,908	421	7,642	665	183	32,747	11,821	2,546	102	227	88	561	.243	.318	.406	.725
16	Red Sox	428	442	.492	870	.508	29,818	4,165	7,668	1,774	115	1,017	3,971	2,674	361	7,665	518	158	33,127	12,723	2,906	57	217	122	609	.257	.324	.427	.750
17	Cardinals	423	445	.487	868	.513	29,049	3,798	7,169	1,409	92	968	3,637	2,746	413	7,009	484	142	32,541	11,666	2,469	104	229	90	577	.247	.318	.402	.720
18	Mets	419	451	.482	870	.518	29,052	3,945	7,208	1,368	107	1,079	3,790	2,804	537	7,145	507	113	32,699	12,027	2,554	99	207	108	627	.248	.324	.414	.738
19	Giants	413	457	.475	870	.525	29,120	3,891	6,988	1,394	128	1,029	3,725	2,960	385	7,746	342	100	32,794	11,725	2,551	95	234	115	611	.240	.316	.403	.719
20	Guardians	406	463	.467	869	.533	28,982	3,676	6,966	1,399	123	866	3,495	2,573	371	6,708	681	170	32,274	11,209	2,388	107	241	158	557	.240	.308	.387	.695
21	Padres	400	470	.460	870	.540	29,175	3,973	7,270	1,437	101	975	3,797	2,985	325	6,679	577	164	32,874	11,834	2,513	163	226	124	623	.249	.323	.406	.729
22	Mariners	400	470	.460	870	.540	28,991	3,841	6,753	1,294	69	1,089	3,684	3,001	507	8,108	616	162	32,764	11,452	2,452	63	202	86	550	.233	.314	.395	.709
23	Blue Jays	398	472	.457	870	.543	29,533	4,138	7,625	1,562	87	1,085	3,976	2,779	330	6,603	429	136	32,925	12,616	2,734	79	204	88	675	.258	.327	.427	.754
24	Phillies	392	478	.451	870	.549	29,402	4,145	7,447	1,451	140	1,115	3,982	2,853	338	7,433	630	141	32,917	12,523	2,706	103	221	130	571	.253	.324	.426	.750
25	Rays	388	482	.446	870	.554	29,264	3,990	7,147	1,458	128	1,000	3,789	2,803	376	7,847	763	230	32,687	11,861	2,586	45	199	81	511	.244	.316	.405	.722