The Montreal Expos had the lowest run differential by a team in 2000, with a differential of -164 runs.

	TEAM	DIFF	SEASON	G	W%	W	L	AB	R	H	2B	3B	HR	RBI	BB	HBP	SO	SB	CS	PA	TB	XBH	SH	SF	IBB	GIDP	AVG	OBP	SLG	OPS
1	Expos	-164	2000	162	.414	67	95	5,535	738	1,475	310	35	178	705	476	29	1,048	58	48	6,152	2,389	523	78	34	53	111	.266	.326	.432	.758
2	Cubs	-140	2000	162	.401	65	97	5,577	764	1,426	272	23	183	722	632	54	1,120	93	37	6,397	2,293	478	89	45	50	114	.256	.335	.411	.746
3	Twins	-132	2000	162	.426	69	93	5,615	748	1,516	325	49	116	711	556	35	1,021	90	45	6,281	2,287	490	24	51	31	143	.270	.337	.407	.744
4	Rangers	-126	2000	162	.438	71	91	5,648	848	1,601	330	35	173	806	580	39	922	69	47	6,363	2,520	538	48	48	39	161	.283	.352	.446	.798
5	Phillies	-122	2000	162	.401	65	97	5,511	708	1,386	304	40	144	668	611	44	1,117	102	30	6,273	2,202	488	70	37	40	115	.251	.329	.400	.729
6	Orioles	-119	2000	162	.457	74	88	5,549	794	1,508	310	22	184	750	558	49	900	126	65	6,237	2,414	516	27	54	34	148	.272	.341	.435	.776
7	Devil Rays	-109	2000	161	.429	69	92	5,505	733	1,414	253	22	162	692	558	51	1,022	90	46	6,206	2,197	437	52	40	25	126	.257	.329	.399	.728
8	Pirates	-95	2000	162	.426	69	93	5,643	793	1,506	320	31	168	749	564	66	1,032	86	40	6,369	2,392	519	59	37	41	133	.267	.339	.424	.762
9	Brewers	-86	2000	163	.451	73	89	5,563	740	1,366	297	25	177	708	620	61	1,245	72	44	6,354	2,244	499	61	49	47	126	.246	.325	.403	.729
10	Marlins	-66	2000	161	.491	79	82	5,509	731	1,441	274	29	160	691	540	60	1,184	168	55	6,202	2,253	463	42	51	39	100	.262	.331	.409	.740
11	Padres	-63	2000	162	.469	76	86	5,560	752	1,413	279	37	157	714	602	46	1,177	131	53	6,290	2,237	473	39	43	50	123	.254	.330	.402	.732
12	Royals	-51	2000	162	.475	77	85	5,709	879	1,644	281	27	150	831	511	48	840	121	35	6,394	2,429	458	56	70	28	139	.288	.348	.425	.773
13	Blue Jays	-47	2000	162	.512	83	79	5,677	861	1,562	328	21	244	826	526	60	1,026	89	34	6,326	2,664	593	29	34	32	130	.275	.341	.469	.810
14	Astros	-6	2000	162	.444	72	90	5,570	938	1,547	289	36	249	900	673	83	1,129	114	52	6,444	2,655	574	57	61	57	154	.278	.361	.477	.837
15	Angels	-5	2000	162	.506	82	80	5,628	864	1,574	309	34	236	837	608	47	1,024	93	52	6,373	2,659	579	47	43	43	126	.280	.352	.472	.825
16	Tigers	-4	2000	162	.488	79	83	5,644	823	1,553	307	41	177	785	562	43	982	83	38	6,340	2,473	525	42	49	22	142	.275	.343	.438	.781
17	Diamondbacks	38	2000	162	.525	85	77	5,527	792	1,466	282	44	179	756	535	59	975	97	44	6,240	2,373	505	61	58	37	114	.265	.333	.429	.763
18	Red Sox	47	2000	162	.525	85	77	5,630	792	1,503	316	32	167	755	611	42	1,019	43	30	6,371	2,384	515	40	48	40	115	.267	.341	.423	.764
19	Yankees	57	2000	161	.540	87	74	5,556	871	1,541	294	25	205	833	631	57	1,007	99	48	6,310	2,500	524	16	50	42	134	.277	.354	.450	.804
20	Reds	60	2000	163	.525	85	77	5,635	825	1,545	302	36	200	794	559	64	995	100	38	6,372	2,519	538	56	58	60	137	.274	.343	.447	.790
21	Dodgers	69	2000	162	.531	86	76	5,481	798	1,408	265	28	211	756	668	51	1,083	95	42	6,312	2,362	504	66	46	42	129	.257	.341	.431	.771
22	Mets	69	2000	162	.580	94	68	5,486	807	1,445	281	20	198	761	675	45	1,037	66	46	6,327	2,360	499	70	51	42	122	.263	.346	.430	.776
23	Rockies	71	2000	162	.506	82	80	5,660	968	1,664	320	53	161	905	601	42	907	131	61	6,453	2,573	534	75	75	64	126	.294	.362	.455	.816
24	Braves	96	2000	162	.586	95	67	5,489	810	1,490	274	26	179	758	595	59	1,010	148	56	6,275	2,353	479	87	45	38	127	.271	.346	.429	.775
25	Cardinals	116	2000	162	.586	95	67	5,478	887	1,481	259	25	235	841	675	84	1,253	87	51	6,369	2,495	519	79	53	33	116	.270	.356	.455	.812